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ORIGINAL ARTICLE
Year : 2011  |  Volume : 2  |  Issue : 3  |  Page : 234-242

The frequency of alloantibodies in pregnant and multi-transfused patients - a comparative study between the ID-Micro Typing Gel System and the conventional tube method


the Blood Bank King Khalid University Hospital King Saud University Riyadh, Kingdom of Saudi Arabia

Date of Web Publication30-Jun-2014

Correspondence Address:
Professor A M Abdel Gader
The Blood Bank King Khalid University Hospital King Saud University P.O. Box 2925 Riyadh 11461
Kingdom of Saudi Arabia
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Source of Support: None, Conflict of Interest: None


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  Abstract 

The DiaMed-ID Micro Typing System (gel test) has become the technique of choice for immune-hematological investigations in blood banks. The aim of this study was to compare the performance of the gel test with the conventional tube low-ionic-strength solution (tube test) technique for the detection of red cell alloantibodies in pregnant women and multi-transfused patients. We prepared sera from the clotted samples of 100 blood donors (controls), 87 pregnant women, 28 ?-thalassemia patients, 34 sickle cell anemia (SCA) patients, and 17 leukemia patients. In the control samples, no antibody was detected with either method. For the pregnant women samples, the most prevalent antibodies detected using the gel test were of the Rhesus type: anti-D (41.4%), anti-E (9.2%), anti-C (5.7%), and anti-Cw (1.1%). We also detected combinations of antibodies against the major Rhesus antigens: anti-C+E (1.1%), anti-E+K (1.1%), anti-C+D (2.3%), and anti-E+S (1.1%). Anti-K was the most prevalent non-Rhesus antibody (18.4%). The gel test also detected other antibodies with minor clinical importance: anti-S (1.1%), anti-M (1.1%), and anti-Lua (1.1%). The gel test also detected a few weak antibodies: anti-D (1.1%), anti-C (2.3%), and anti-E (1.1%). In the same samples, the tube test detected the following antibodies: anti-D (25.3%), anti-E (5.7%), anti-K (12.6%), anti-C (2.3%), non-specific (9.2%), anti-C+D (1.1%), anti-Fya (1.1%), and weak anti-K (1.1%). For the -thalassemia samples, only the gel test detected a single antibody (anti-Kpa). For the SCA samples, the gel test detected one anti-K (2.9%)antibody and 2 antibody combinations: anti-D+K+C (2.9%) and anti-E+K (2.9%). The tube test also detected anti-K (2.9%); however, for the anti-D+K+C combination, it only detected anti-C (2.9%), while it only detected anti-K from the anti-E+K combination. For the leukemia samples, both methods gave negative reactions. In conclusion, the gel test is more sensitive than the tube test at detecting alloantibodies; moreover, it is technically easier to perform, with clear readable agglutination reactions, requires small volumes of sera and reagents, and has no washing steps.


How to cite this article:
Hassan MA, Gahtani F, Abdel Gader A M. The frequency of alloantibodies in pregnant and multi-transfused patients - a comparative study between the ID-Micro Typing Gel System and the conventional tube method. J Appl Hematol 2011;2:234-42

How to cite this URL:
Hassan MA, Gahtani F, Abdel Gader A M. The frequency of alloantibodies in pregnant and multi-transfused patients - a comparative study between the ID-Micro Typing Gel System and the conventional tube method. J Appl Hematol [serial online] 2011 [cited 2022 Jul 5];2:234-42. Available from: https://www.jahjournal.org/text.asp?2011/2/3/234/135592

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