Year : 2018 | Volume
: 9 | Issue : 2 | Page : 45--50
Discrepancies between DNA index by flow cytometry and cytogenetic studies in childhood B-Lymphoblastic leukemia
Nour Almozain1, Ayman Mashi2, Suleimman Al-Sweedan3, Asim Belgaumi4, Faisal Rawas5, Randa Alnounou5
1 Division of Hematology, Department of Pathology, King Saud University Medical City, Riyadh, Saudi Arabia
2 Hematology Section, Pathology Department, King Fahad Central Hospital, Ministry of Health, Jazan, Saudi Arabia
3 Pediatric Hematology/Oncology, King Faisal Specialist Hospital & Research Centre, Riyadh, Saudi Arabia
4 Division of Hematology Oncology, Sidra Medical and Research Center, Doha, Qatar
5 Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia
BACKGROUND: DNA index by flow cytometry (DNAI-FCM) is a rapid technique used in classification of acute B-lymphoblastic leukemia (B-ALL). The objective of this study is to estimate the reliability of FCM in the early classification of childhood B-ALL and to analyze the causes of discrepancies between the DNAI-FCM and the cytogenetic studies (CG) (Karyotype and Fluorescent in situ Hybridization [FISH]).
MATERIALS AND METHODS: DNAI-FCM and CG (Karyotype and FISH) were analyzed in 69 consecutive children, newly diagnosed with B-ALL in King Faisal Specialist Hospital and Research Centre between January 2013 and June 2014.
RESULTS: A statistically significant correlation existed between DNAI-FCM and CG (P = 0.001). DNAI-FCM was proportional to CG in 82.6% (57/69) of the cases. There was a discrepancy between the DNAI-FCM and the CG in 17.4% (12/69) of the cases.
CONCLUSION: DNAI-FCM shows 82.6% concordance with CG in childhood B-ALL with a predictive value of 81%. Discrepancies occur due to either the small size of the chromosome or due to insufficient genetic material representing the abnormality.
Dr. Randa Alnounou
Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital, Riyadh
|How to cite this article:|
Almozain N, Mashi A, Al-Sweedan S, Belgaumi A, Rawas F, Alnounou R. Discrepancies between DNA index by flow cytometry and cytogenetic studies in childhood B-Lymphoblastic leukemia.J Appl Hematol 2018;9:45-50
|How to cite this URL:|
Almozain N, Mashi A, Al-Sweedan S, Belgaumi A, Rawas F, Alnounou R. Discrepancies between DNA index by flow cytometry and cytogenetic studies in childhood B-Lymphoblastic leukemia. J Appl Hematol [serial online] 2018 [cited 2020 Jan 23 ];9:45-50
Available from: http://www.jahjournal.org/article.asp?issn=1658-5127;year=2018;volume=9;issue=2;spage=45;epage=50;aulast=Almozain;type=0