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ORIGINAL ARTICLE
Year : 2013  |  Volume : 4  |  Issue : 3  |  Page : 104-109

Study of hemoglobinopathies and Hb variants in population of Western India using HPLC: A report of 7,000 cases


1 Department of Pathology, C. U. Shah Medical College, Surendranagar, Gujarat, India
2 Department of Hematology, Green Cross Pathology and Molecular Laboratory, Ahmedabad, Gujarat, India

Correspondence Address:
Atul Shrivastav
Department of Pathology, C. U. Shah Medical College, Dudhrej Road, Surendranagar, Gujarat 363 001
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1658-5127.123308

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Context : Various hemoglobinopathies are one of the major public health problems of India. Aims: High performance liquid chromatography (HPLC) is a speedy and accurate tool for diagnosis of various hemoglobinopathies. In present study about 7,000 cases have been studied for identification of various hemoglobin (Hb) disorders in western India. Settings and Design : Study is conducted from May 2010 till April 2013 for various hemoglobinopathies and variants. The geographical distribution of all cases included states of western India. Materials and Methods: Complete blood count (CBC) is done on CELL DYN 3700 analyzer and then HPLC is performed on BIO-RAD 'VARIANT II' (beta thalassemia short program) in samples received in our laboratory. Simple Statistical analysis is done with help of Microsoft Office Excel 2007. Results: A total of 7,261 cases were included in our study, out of these 1,615 (22.24%) cases showed abnormal Hb fractions. The major abnormality observed was of high HbA2, a cutoff value of > 3.9% was considered for diagnosis of beta thalassemia trait (BTT). A total of 839 cases (11.55%) of BTT were diagnosed. Other hemoglobinopathies were also identified in varying proportions. Conclusions: HPLC is simple, accurate, and superior technique combined with complete automation makes it an ideal method for diagnosis of hemoglobinopathies.


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